Neoplasma Vol.52, p.79-83, 2005
|
| Title: Chromosome damage induced by benzene after the use of conventional
and FISH chromosome painting
|
| Author: K., SIVIKOVA
; B., HOLECKOVA
; J., DIANOVSKY
; |
|
Abstract: The cytogenetic effects of in vitro exposure to benzene were
investigated in cultured bovine peripheral lymphocytes. Stable and
unstable chromosome aberrations (CA) and sister chromatid
exchanges (SCE) were examined. Cultures of lymphocytes from two
healthy donors were treated with benzene at concentrations of 5,
10, 50, 100, 500 and 1000 Ţmol.l-1 for the last 24 hours and for 2
hours of cultivation, both without and with metabolic activation
(S9). In the CA assays with conventional Giemsa staining for 24
hours without metabolic activation, no dose-dependence in the
induced chromosome aberration was achieved. A significant
elevation in the induction of CA was found only after the
application of benzene at a dose of 100 Ţmol.l-1 (p<0.01). Similar
results were observed using fluorescence in situ hybridization
(FISH) method for the detection both of non-stable and persistent
chromatid-type aberrations and in the SCE assay for 24 hours
without S9. All the lower and higher concentrations tested have no
positive influence on CA and SCE induction. In experiments with S9
a significant increase in CAs, and a dose-dependence was achieved
after exposure to benzene for 2 hours at concentrations ranging
from 50 to 500 Ţmol.l-1 (p<0.05 and p<0.01, respectively).
|
|
| Keywords: : benzene, bovine peripheral lymphocytes, chromosome aberrations,
sister chromatid exchanges, FISH
|
|
|
|
| Year: 2005, Volume: 52, Issue: |
Page From: 79, Page To: 83 |
|
|
 download file |
|
|
|
|
