Download full text in PDF format (117 kB)Electronic Library of Scientific Literature - © Academic Electronic Press
Volume 39 / No. 2 / 2005
Original articles
Review
Endocrine Regulations (since 1967 to 1990 Endocrinologia Experimentalis) is an international journal on experimental and clinical endocrinology edited quarterly in English by care of the Institute of Experimental Endocrinology, Slovak Academy of Sciences (Bratislava, Slovakia) and published by the Slovak Academic Press (Bratislava, Slovakia).
This journal aims to publish original manuscripts or minireviews on experimental and clinical endocrinology and diabetes.
The submission of a manuscript to Endocrine Regulations implies that it has not been previously published or is not being submitted for publication elsewhere and that the manuscript has been approved by all authors who are ready to take public responsibility for the content.
All materials relating to human investigation will be published upon the understanding that design of the work has been approved by the local Ethical Committee or that it conforms to ethical guidelines of the Declaration of Helsinki. The animal experiments should state the conformance to guidelines on animal care.
Manuscripts in triplicate with three sets of illustrations (of which one is an original) should be sent to:
Richard Kvetnansky, Ph.D., Dr.Sc., Chief Editor,
Institute of Experimental Endocrinology,
Vlárska 3, 833 06 Bratislava, Slovakia
All text must be printed on one side of the sheet only with appropriate margins and double spacing to give adequate space for editorial notes. The corresponding author should indicate his/her full mailing address including phone and fax numbers and the e-mail address.
Manuscripts on disc. The submissions of manuscripts prepared on 3.5 inch discs on IBM compatible computers is encouraged, the preferred word processors being Microsoft Word. However, also in this case the disc must be accompanied by three hard copies of the manuscript. The disk should be labelled by the name of the first author, type of word processor, its version and file name and must also accompany the final version of the manuscript.
Types of manuscripts. Standard original papers should contain following sections: * Title, * Abstract (divided into sections Objective, Methods, Results, Conclusions), * Key Words, * Introduction, * Materials and Methods (in clinical papers this section should read * Subjects and Methods), * Results, * Discussion, (* Acknowledgements), * References. There is no length limit for these papers.
Minireviews should give an overview of a defined field preferably of author,s own professional interest and experience. They should not exceed 25 typed pages including complete References and should usually contain * Abstract, * Key Words, * Individual sections and subsections, * References.
Title page should give * the title of the article (main key words should be preferably included into the title to give sufficient information to allow the reader to judge the relevance of a paper to his field), * full names of authors, * institute of origin, * short title (running head), * name and full address of corresponding author including phone and fax numbers and e-mail naddress as well.
Abstract should clearly indicate the purpose of the study (Objective), basic procedures (Methods), main findings (Results) and principal conclusions (Conclusions). New and original findings should be emphasized, clearly defined and defended. The abstract must be easily understood indepenently of the full text of the paper
Key Words. Up to 8 key words (in exceptional cases even more) should be carefully selected to give appropriate information to the users of international information networks.
Introduction should give a brief overview of background informations and clearly define the purpose of the study...
Materials and Methods (in clinical manuscripts Subjects and Methods) should give full informations sufficient to allow others to repeat the work. It is recommended to divide it into subsections. Established and routine methods (if not considerably modified) should be just cited by the appropriate references, the modifications being briefly but clearly described. Statistical methods should be clearly described.
Results should describe concisely and clearly the results in logical sequence. Any interpretations should be avoided and definitely shifted to the Discussion. Do not repeat Materials and Methods, and do not repeat the data presented in tables and figures.
Discussion. Do not simply repeat the data presented in Introduction and Results section. Define and emphasize the new and important aspects of the study and the conclusions that follow. Relate results to other relevant studies, interpret them and explain the differences, if any. Working hypotheses and theories may be briefly outlined.
Acknowledgements. This short section, if necessary, contains acknowledgements of personal and/or financial assistance.
References. Begin this section on a new page. References should be assembled in alphabetical order according to the first author. More than one paper from the same author(s) in the same year must be identified by the letters a, b, c etc. placed after the year of publication. All listed references must be cited in the text by the first author et al. and the year (in a case of two authors only cite both). Following possibilities are recommended: (1) Brown and White (1993) found that ...; (2) ... as observed by Black et al. (1992); (3) ... as previously reported by several authors (Black et al. 1992; Brown and White 1993; Green et al. 1995).
The names of authors in the text and in references should be typed in small letters and underlined (e.g. White and Brown). The volume should be typed in bold.
The style for the list of references is as follows:
A.Journal Articles:
Itoh M, Okugawa T, Shiratori N, Ohashi H: Treatment with triiodothyronine (T3) against multinodular goiter fails to prevent the onset of Graves disease. Endocrine Regul 29, 151-156, 1995B. Book Chapters:
Mornex R, Orgiazzi JJ: Hyperthyroidism. In: The Thyroid Gland (Ed. M de Visscher), pp. 279-362, Raven Press, New York 1980
C. Books:
Podoba J: Endemic goiter in Slovakia. VEDA, Bratislava, 1962
The statement “in press” may be used only for a paper accepted for publication in the indicated journal. Unpublished data or Personal communication may be used in the text, but must not be listed in References.
Tables should be constructed as simply as possible, typed on separate sheets and numbered consecutively with Arabic numeral. There should be a short and descriptive heading and appropriate footnotes. Not more than 4 vertical rows should be used in a table planned to occupy one column and not more than 8-10 rows for that designed for two columns of a page.
Figures should be prepared in proportional way with lettering of appropriate size in order to permit such reduction in size to occupy either one or two columns on the page. Drawings (graphs, charts, diagrams etc.) should be submitted either as original or camera ready glossy photographs. Computer generated graphs must be printed by high quality laser printers on high quality camera ready paper. High quality photographs should be submitted on glossy paper.
Units of Measurement. Results should be expressed in SI units.
Abbreviations. Non-standard abbreviations should be properly defined in the text the first time they are used.
There are no page charges. Reprints order forms are sent to the corresponding author together with galley proofs. Color illustrations may be published for extra charges.
Download full text in PDF format (117 kB)
EWA LUCIA GREGORASZCZUK1, MALOGORZATA ZEMLA1, ANNA PTAK1, ROMAN GRABIC2
1 Laboratory of Physiology and Toxicology of Reproduction, Department of Animal Physiology, Institute of Zoology,
Jagiellonian University, Kraków, Poland;
2 Institute of Public Health in Ostrava, National Reference Laboratory for Analysis of POPs, Ministry of Health CR, 702 00 Ostrava, Czech Republic, e-mail: greg@zuk.iz.uj.edu.pl
Summary:
Objective. To compare the effects on ovarian function of low chlorinated polychlorinated biphenyls (PCBs) mixture generally distributed at a faster rate and metabolised to a greater extent (Delor 103) with high chlorinated PCBs mixture which are accumulated in large amounts in biological samples (Delor 106).
Methods. Theca interna and granulosa cells were isolated from prepubertal porcine follicles and initially cultured in M199/CS without test compounds for 24 hrs to allow for cell attachment to the wells. After 24 h, the medium was discarded and 0.3 ml of fresh M199 alone was added to the control culture, while Delor 103 or Delor 106 at concentrations of 0.02, 0.2 or 2 ng/ml medium were added to the cultures. For analysis of steroid levels, the media were collected after 24 hrs (single exposure) or 72 hrs (repeated exposure) and frozen at – 20 0C. Additionally, after 72 hrs, LDL test was used to show the action of both mixtures on the cell viability and cells were used for the measurement of caspase-3 activity.
Results. An increase in testosterone secretion after the single exposure and strong stimulatory action on estradiol secretion was noted after permanent treatment with Delor 103. In Delor 106
treated cultures, antiestrogenic action after the single exposure and strong estrogenic action in permanently treated cells was observed. Both mixtures had no effect on cell viability and apoptosis.
Conclusions. By stimulation of estradiol secretion from ovarian follicles in prepubertal animals, PCB mixture could be responsible for premature development of follicles and in consequence, premature sexual maturation.
Key Words: Delor-103 - Delor-106 - Prepubertal ovary - Steroid secretion - Cell viability - Cell apoptosis
ENDOCRINE REGULATIONS, VOL 39, 33-41, 2005
Download full text in PDF format (3,04 MB)
ELENA LIPSKA1, MARTA NOVOTOVA1, TATIANA RADZYUKEVICH2, IVAN ZAHRADNIK1
1 Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovak Republic;
2 I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St.-Petersburg, Russian Federation, E-mail: umfgnovo@savba.savba.sk
Summary:
Objective. The aim of the present study was to characterise and compare alterations in the ultrastructure of the functionally identified isolated twitch skeletal muscle fibres of the frog after repeated tetanic stimulation and under experimental conditions which modified their fatiguability.
Methods. Single isolated twitch muscle fibres of m. iliofibularis of adult frogs Rana temporaria were subjected to intermittent tetanic stimulation. Fibres at specified degree of fatique were processed for electron microscopic observation and ultrastructural examination.
Results. The fatigue-resistant (FR) fibres that developed 90 % of the control tetanic tension after 10 min stimulation in ordinary Ringer’s solution showed regions with dilated intermyofibrillar spaces containing small vesicles and swollen mitochondria. In addition to the changes observed in FR fibres, the easily fatigued (EF) fibres that produced 50 % of the original tension after 3 min stimulation showed small vacuoles in the sarcoplasm. In EF fibres that preserved 10 % of the control tension after 10 min stimulation and showed swelling of the longitudinal sarcoplasmic reticulum, the central element of triads and mitochondria, large vacuoles were present. FR fibres exposed to low Ca2+ medium containing 0.02 mmol/l verapamil, lost their resistance to fatigue. Their contractile responses fell down to 20 % within 0.5 min of stimulation. Those fibres displayed large vacuoles and changes in mitochondria as observed in EF fibres after 10 min stimulation.
Conclusion. These results suggest that morphological changes accompanying reduction of the contractile force (i) appear earlier than the reduction of the contractile ability, (ii) correlate with the degree of reduction of the contractile capacity but not with the duration of contractile activity, (iii) are not specific for the fatique fibre type.
Key words: Skeletal muscle – Fatigue – Ultrastructure – Verapamil – Calcium
ENDOCRINE REGULATIONS, VOL. 39, 43-52, 2005
Electronic Library of Scientific Literature - © Academic Electronic Press