Electronic Library of Scientific Literature
Volume 42 / No. 2 / 1995
Sialylated Lewis[x] and Lewis[a] determinants expression on human neoplastic cell lines: immunocytometric study with the 5th workshop monoclonal antibodies
J. SEDLAK, L. HUNAKOVA, M. SULIKOVA, B. CHORVATH
Cancer Research Institute, Slovak Academy of Sciences, 812 32 Bratislava, Slovakia
The reactivity of monoclonal antibodies from the 5th workshop Selectins/Selectin Ligands panel, directed to the sialylated Lewis[x] and sialylated Lewis[a] determinants, with the human breast carcinoma (BT-20, ZR-75-1 and MDA-MB-468) cell lines, human ovarian carcinoma cell line A2780, fibrosarcoma (HT-1080, B-6FS) and hematopoietic neoplastic (U-937, HL-60, K-562) cell lines were determined with the aid of flow immunocytofluorometry. The examined monoclonal antibodies to sialylated Lewis determinants reacted with examined breast carcinoma, but not with the examined ovarian carcinoma and fibrosarcoma cell lines.
Key words: SLe[x], Sle[a], monoclonal antibodies, breast carcinoma, ovarian carcinoma, fibrosarcoma, melanoma, HL-60, U-937, K-562 cell lines.
Activities of enzyme transducing extracellular signals - gamma glutamyltransferase and enzymes metabolizing glutathione in acute lymphoblastic and myeloid human leukemias
Department of Cell Biology, University of M. Sklodowska-Curie, 20 - 033 Lublin, Poland
The ectoenzyme gamma glutamyltransferase (GGT) a second messenger generating enzyme activity on the cytoplasmic membrane was biochemically analyzed in leukemic cells from patients with acute lymphoblastic and myeloid leukemias.
The lower mean activity - 0.594 IU/mg protein was noticed in patients with acute lymphoblastic leukemias (ALL), while the higher - 0.956 IU/mg protein was found in acute myeloid leukemia patients (AML) in serum and 0.151 IU/mg protein in polymorphonuclear cells. The levels of the activity of glutathione reductase (GR) were increased but the activities of glutathione peroxidase (GSH Px) were significantly decreased in serum of leukemia patients.
Key words: gamma glutamyltransferase, glutathione reductase, glutathione peroxidase, acute lymphoblastic leukemia, acute myeloid leukemia.
Hormonal regulation of adenylate cyclase activity in circulating lymphocytes and its interrelationship with hormone sensitivity of tumor tissue in colorectal cancer patients
L.M. BERSTEIN, I.V. PRAVOSUDOV, O.G. KRYUKOVA
N.N. Petrov Research Institute of Oncology, St. Petersburg, 189 646 Russia
The purpose of this study was to investigate the peculiarities of hormonal regulation of adenylate cyclase (AC) of blood lymphocytes in colorectal cancer patients and to compare these peculiarities with hormone sensitivity of AC of colorectal tumors and normal colonic mucosa. Basal and stimulated lymphocyte AC activity was studied in 51 healthy persons and 52 cancer patients (14 with colon cancer, 21 with rectal cancer and 17 with stomach cancer) aged 20-75 years. In 31 of 35 patients with colorectal cancer the AC activity was studied simultaneously in lymphocytes, tumor tissue and normal colonic mucosa. To evaluate basal and stimulated AC activity the measurement of c-AMP (Amersham kits) formed in the presence of ATP regenerating system was used. Basal and by VIP, pentagastrin and sodium fluoride stimulated AC activity in lymphocytes of gastrointestinal cancer patients was lower than in lymphocytes of healthy subjects of similar age. Stage dependence of the parameters under study was not found. There was a tendency for higher basal and stimulated lymphocyte AC activity in colon cancer patients as compared to stomach and rectal cancer patients. In colorectal cancer patients the peculiarities of lymphocyte AC reactions to stimulation were closer to those in tumor tissue but not to those in normal colonic mucosa. The reaction of lymphocyte AC to VIP and glucagon coincided more frequently with tumor AC reactions to the same hormones in case of hormone nonsensitive tumors.
Thus, basal and stimulated lymphocyte AC activity in colorectal cancer patients was modified to some degree by tumor factors. Lymphocyte AC reactions to VIP and glucagon may be considered as indirect markers of hormone sensitivity of colonic tumors. Moreover, the probability of discovery of hormone nonsensitive tumors by this way is more reliable than hormone sensitive ones.
Key words: Adenylate cyclase, blood lymphocytes, large bowel cancer, stomach cancer, colorectal tumor tissue, hormone sensitivity.
Influence of liposomal muramyl tripeptide phosphatidylethanolamine on the changes of nucleic acids in the blood and hemopoietic tissues of irradiated mice
E. MISUROVA, P. FEDOROCKO
Department of Cellular and Molecular Biology, Faculty of Science, P.J. Safarik University, 041 67 Kosice, Slovakia
Effects of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE/MLV) on nucleic acid content, and on DNA synthesis and cellularity in the blood and haemopoietic tissues of mice irradiated with a dose of 6 Gy gamma radiation were studied.
It was found that injection of MTP-PE/MLV alone (200 microg per mouse; i.p.) resulted in a mild decrease in DNA content in the bone marrow as consequence of cellularity diminution in the femur. At the same time, incorporation of H-thymidine into bone marrow DNA increased in comparison to the non-stimulated mice. On the basis of simultaneous increase in RNA and DNA concentration in the blood we assume that the changes of nucleic acids and cellularity in the bone marrow of MTP-PE/MLV treated mice are related to the higher release of nuclear cells into the circulation. In mice irradiated 24 h after MTP-PE/MLV injection the decrease in nucleic acid content was similar to that in unprotected mice within the first three days after irradiation. However, during following days, the recovery of RNA and DNA content and cellularity in the bone marrow and blood proceeded at a much faster rate in mice protected by MTP-PE/MLV as compared to unprotected animals. The increase in DNA synthesis in the bone marrow of protected mice preceded the DNA content recovery. In the spleen and the thymus, no effect of liposomal MTP-PE was found.
Key words: Irradiation, liposomal MTP-PE, DNA, RNA, mice, blood, bone marrow.
Synergistic antitumor activity of tumor necrosis factor-alpha and lovastatin against MmB16 melanoma in mice
W. FELESZKO, W. LASEK, J. GOLAB, M. JAKOBISIAK
Department of Immunology, Institute of Biostructure, Medical School, PL-02-004 Warsaw, Poland
Lovastatin, the drug introduced recently to treat hypercholesterolemia and displaying antiproliferative activity against tumor cells in vitro, was used for the local therapy of MmB16 melanoma in mice. Female BDF mice were injected with 1x1 000.000 of MmB16 melanoma cells into the right hind limb. On the 7th day after the injection of tumor cells mice were divided into four groups and were injected with: (a) saline solution (control group), (b) TNF-alpha alone, (c) lovastatin alone, and (d) a combination of TNF-alpha and lovastatin. Statistically significant inhibition of tumor growth was observed in mice treated with both TNF (5 microg/day) and lovastatin (200 microg/day). We also observed the prolongation of survival of tumor-bearing mice after combined therapy with both TNF-alpha (5 microg/day) and lovastatin (1 mg/day) in comparison to all other groups. Our data suggest that lovastatin may synergistically potentiate the antitumor activity of TNF-alpha.
Key words: Tumor necrosis factor-alpha, lovastatin, tumor therapy, ras-oncogene.
The release of TNF-alpha (factor) by peritoneal macrophages of hamsters bearing transplantable melanomas
K. KOZLOWSKA, M. CICHOREK
Laboratory for Cytophysiology, Department of Histology and Immunology, Medical School, 80-211 Gdansk, Poland
The influence of two kinds of transplantable melanomas on the secretory function of peritoneal macrophages, and particularly the release of TNF-alpha has been studied. The results showed a statistically significant increase of protein content in the supernatants from 24 h cultured macrophages from melanoma-bearing hamsters in comparison with control macrophages.
The release of TNF-alpha by control macrophages was higher than that by macrophages of hamsters with transplantable melanomas. The decrease in release of this factor was more prominent in case of macrophages from hamsters bearing amelanotic melanoma, it may suggest that biological features of melanomas can influence peritoneal macrophages to release the TNF-alpha at different level.
Key words: TNF-alpha, peritoneal macrophages, transplantable melanomas.
Brick mortar exposure and chronic lymphocytic leukemia
Lj. MARKOVIC-DENIC, S. JANKOVIC, J. MARINKOVIC,¹ Z. RADOVANOVIC²
Institute of Epidemiology, Faculty of Medicine, Belgrade, Yugoslavia;
¹Institute of Social Medicine, Statistics and Health Research, Faculty of Medicine, Belgrade, Yugoslavia;
²Department of Community Medicine and Behavioural Sciences, Faculty of Medicine, 13 110, Safat, Kuwait
A case-control study of 130 patients with chronic lymphocytic leukemia (CLL) and 130 controls matched with respect to sex, age (2 years), type of residence (urban-rural) and area of residence (according to the national per capita income) was carried out. Conditional logistic regression analysis showed that, apart of four risk factors already described in the literature (work in a hazardous industry, hair dye use, family history of leukemia and exposure to electromagnetic radiation), brick mortar exposure was also significantly related to CLL.
Key words: Epidemiology, risk factors, occupational hazard, brick mortar, hair dye, family history, electromagnetic radiation.
Evaluation of biometric parameters of Morris hepatoma after application of human recombinant tumor necrosis factor
S. TERLIKOWSKI, H.F. NOWAK,¹ W. LOTOCKI
Department of Gynecology and Septic Obstetrics and
¹Department of Pathological Anatomy, Medical School, Bialystok 15-062, Poland
The effect of human recombinant tumor necrosis factor alpha (h rec TNF-alpha) on the growth of Morris hepatoma 5123 implanted in the skeletal muscles of the thigh of Buffalo rats was investigated. The cytokine was repeatedly given in an intratumor administration (i.t.) in dose of 1.5x10.000 U once a day in regimens of four or eight days. Comparative groups consisted of animals which were given saline i.t. Control groups included healthy rats subjected to local cytokine effect. The experiments revealed an inhibitory effects of the preparation on the growth of tumors. Biometric parameters of the tumors induced indicated that the inhibition of Morris hepatoma was most effective after the eighth dose of h rec TNF-alpha. The administration of fourfold dose resulted in an initial loss of body mass increase. However, when injected eight times, the factor produced a relative tolerance reflected in minor reduction of actual body mass. The estimation of survival time in rats injected i.t. with h rec TNF-alpha, compared to those given saline, revealed statistically significant differences at the eighth repeated dose.
Key words: Human recombinant tumor necrosis factor (h rec TNF-alpha), Morris hepatoma 5123, tumor growth.
Hepatic perfusion index in evaluating treatment effect of transcatheter hepatic artery embolization in patients with hepatocellular carcinoma
W.Y. LIN, S.J. WANG, S.H. YEH
Department of Nuclear Medicine, Taichung Veterans General Hospital, Taichung 407, Taiwan
We assumed the hepatic perfusion index (HPI) in patients with hepatocellular carcinoma (HCC) treated by transcatheter arterial embolization (TAE) and compared the results with the following CT findings.
From September 1993 to February 1994, 15 patients with newly diagnosed HCC, proven by biopsy, were studied. Hepatic perfusion index (HPI) studies were performed before-TAE as well as on the 1st day and 7th day post-TAE, and CT scans were performed before and one month after the TAE. HPI at 1st-day post-TAE (HPI1) over HPI pre-TAE (HPIp) and HPI at 7th-day post-TAE (HP17) over HPIp were calculated. The HPI7/HPIps were chosen to evaluate the efficacy of TAE because they had better correlation with the CT findings than HPI1/HPIps. CT scans performed one month after the TAE showed obvious reduction of tumor size in all 7 patients with a HP17/HPI p <0.85 but in only 2 of the 7 patients with a HP17/HPI p >= 0.85. The difference was significant, with a p-value of 0.01 by Fisher's exact test.
We consider that the HPI with its characteristics of relative safety, convenience, low radiation exposure, and inexpense, may provide an useful modality for early prediction of the efficacy of hepatic artery embolization in the treatment of HCC.
Key words: Hepatic perfusion index, hepatocellular carcinoma, transcatheter hepatic artery embolization.
Evaluation of a hospital based cytology screening programme for reduction in life time risk of cervical cancer
S.S. AGARWAL, N.S. MURTHY, SHASHI SHARMA, K.C. SHARMA, D.K. DAS
Institute of Cytology and Preventive Oncology (ICMR), Maulana Azad Medical College Campus, New Delhi-110 002, India
Hospital based cytology screening is one of the suggested alternative strategies for the developing countries. The present communication attempts to estimate the reduction in lifetime risk of cervical cancer initiated through a hospital based single lifetime screening programme. The percent reduction in cumulative incidence of cervical cancer during lifetime in different age groups of women was calculated after estimating the number of incident cases in the absence as well as presence of screening. Our analysis revealed that by introducing the single life time cytology screening in the group of hospital attending population, an overall reduction in the cumulative incidence of cervical cancer during lifetime was found to be 10.2%. It was further estimated that the reduction was much less in the early age groups (2.4-10.2% in 20-34 years) as compared to later age groups (11.2-55.6% in 35+ years).
Key words: Uterine cervix cancer screening, hospital based screening, life time risk.