Electronic Library of Scientific Literature
Electronic Library of Scientific Literature
Volume IX / 1995, Number 4
THE EFFECT OF BIOMIN-H ON IN VITRO GROWTH CHICK EMBRYONIC CARTILAGE. ENZYMHISTOCHEMICAL STUDY
D. PAVLIKOVA¹, J. ROVENSKY², J. MAREK³, O. SCHREIBEROVA³, E. ROVENSKA¹
¹Ustav histologie a embryologie LFUK, Bratislava
Prednosta: doc. MUDr. J. Zlatos, DrSc.
²Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
³III. interni klinika I. LF UK, Praha
Prednosta: prof. MUDr. J. Marek, DrSc.
Suhrn
V predchadzajucom studiu biologickych vlastnosti Biominu-H (biopreparatu
pripraveneho zo skrupin slepacich vajec) sa zistilo, ze alkalicka
fosfataza (AF), ktora je markerom diferenciacie chondrocytov a
osteoblastov, priamo odraza ucinok tohto biopreparatu na bunky
a tkaniva. Pomocou histochemickej azokopulacnej metody na dokaz
alkalickej fosfatazy sme sledovali rast a diferenciaciu embryonalnych
chrupiek kurcata (panvovej chrupky, femuru a tibie) pestovanych
in vitro za pritomnosti Biominu-H. Chrupky 9-dnovych kuracich
embryi boli inkubovane 24 h (A) a 48 h (B) v mediu s Biominom-H
a v mediu samotnom, ktore sluzilo ako kontrola. Najvyraznejsie
sa odrazil ucinok Biominu-H na lokalizaciu a aktivitu AF po 24-hodinovej
inkubacii. V centralnej casti panvovej chrupky sa nachadzalo velke
mnozstvo okruhlych a hypertrofickych chondrocytov s pozitivitou
AF v bunkovej membrane. V chrupke femuru a tibie sa vrstva AF
pozitivnych, diferencujucich sa a hypertrofickych chondrocytov
vyrazne rozsirila oproti kontrole. Po 48-hodinovej inkubacii s
Biominom-H aktivita AF aj pocet pozitivnych chondrocytov mierne
klesli, ale naopak aktivita a pocet AF pozitivnych buniek v kontrole
mierne stupli. Aktivita lyzozomalnych enzymov: kyslej fosfatazy
a beta-D-galaktozidazy mierne stupla po 24-hodinovej inkubacii
s Biominom-H. O vyskyte vysokej aktivity AF v tkanive inkubovanom
s Biominom -H sa diskutuje vo vztahu k Ca[^2+]-ionom a somatomedinovej
aktivite, ktore sa nachadzaju v tomto biopreparate. Mozeme uzavriet,
ze Biomin-H aktivuje enzymy diferencujucich sa chondrocytov, stabilizuje
ich bunkove membrany a aktivuje v nich vyskyt AF, cim umoznuje
rast chrupky v podmienkach in vitro.
Klucove slova: Biomin-H, alkalicka fosfataza, lokalizacia,
diferenciacia, chondrocyty, embryonalna chrupka kurcata, Ca[^2+]-iony.
Summary
The previous study of biological features of Biomin-H (biopreparation
prepared from chicken egg shells) has shown that alkaline phosphatase
(AP), which is the marker of differentiation of chondrocytes and
osteoblasts, directly reflected the effect of this biopreparation
on cells and tissues. By means of histochemical azocoupling method
for detection of alkaline phosphatase we have studied in vitro
growth of chick embryonic cartilage (pelvic cartilage, femur and
tibia) observing the differentiation of chondrocytes. Cartilages
of 9-day old chick embryos were incubated 24 h (A) and 48 h (B)
in medium with Biomin-H and in medium alone as a controle. The
most conspicuous effect of Biomin-H was obsereved after 24 hours
incubation. In pelvic cartilage there were masses of rounded and
hypertrophied chondrocytes with very strong AP activity in cell
membrane in the central part. In comparison with controle, the
zone of rounded and hypertrophied AP positive chondrocytes was
increased in femur and tibia. The activity of enzyme and the amount
of AP positive chondrocytes was decreased after 48 hours incubation
with Biomin-H. On the contrary, the AP activity and the amount
AP positive chodrocytes slowly increased in the controle. Not
only AP activity but also some lysosomal enzymes (acid phosphatase
and beta-D-galaktosidase) were moderately increased after 24 hours
incubation with Biomin-H. The appearance of high AP activity after
24 hours incubation with Biomin-H is discussed in connection with
Ca[^2+]ions and somatomedin activity, both detected in this biopreparation.
We can conclude that Biomin-H activates enzymes of differentiating
chondrocytes, stabilizes cell membranes and activates their AP
and so it enables in vitro growth of cartilage.
Key words: Biomin-H, alkaline phosphatase, localization,
chondrocytes differentiation, chick embryonic cartilage, Ca[^2+]-ions.
pp. 185-192
COMPARISON OF THE INFLUENCE OF NON-STEROIDAL ANTIREUMATIC DRUGS ON BIOSYNTHESIS OF CERTAIN COMPONENTS OF EXTRACELLULAR MATRIX BY CHONDROCYTES FROM NORMAL AND OSTEOARTHROTIC CARTILAGE
J. KRAJICKOVA, R. VYTASEK, K. TRNAVSKY
Revmatologicky ustav, Praha
Reditel: doc. MUDr. K. Pavelka, CSc.
Souhrn
Studovali jsme metabolismus mimobunecne matrix v jednovrstevnych
kulturach chrondrocytu (monolayer) lidske normalni a osteoartroticke
chrupavky. Inkorporace radioaktivne znaceneho sulfatu a prolinu
byl snizen v osteoartroticke chrupavce behem celeho tritydenniho
sledovani. Pridani indometacinu, diclofenaku, kyseliny tiaprofenove
a tropesinu (tropoveho esteru indometacinu) do inkubacniho media
po tri tydny nevedlo k zadnym vyznamnym zmenam v metabolismu chondrocytu.
Zadne ze studovanych nesteroidnich antirevmatik nesnizilo vyznamne
metabolickou aktivitu v kulturach chondrocytu osteoartroticke
chrupavky. Indometacin v koncentraci 10[^-5] a 10[^-6] mol/l zvysil
miru inkorporace. Kyselina tiaprofenova a tropesin v nejnizsi
koncentraci 10[^-7] mol/l (blizici se terapeuticke hladine) vyznamne
zvysily inkorporaci znaceneho sulfatu a prolinu do chondrocytu
z osteoartroticke chrupavky.
Klicova slova: nesteroidni antirevmatika, biosynteza, mimobunecna
matrix, chondrocyty, osteoartroticka chrupavka.
Summary
We have studied metabolism of extracellular matrix in monolayer
chondrocyte cultures of human normal and osteoarthrotic cartilage.
Incorporation of radioactively stained sulphate and proline was
decreased in the osteoarthrotic cartilage during the whole three-week
study. Adding of indometacin, diclofenac, tiaprofenic acid and
tropesin (tropic ester of indometacin) into the incubation medium
during three-weeks has not resulted in any important changes in
the chondrocyte metabolism. None of the non-steroidal antirheumatic
drugs studied reduced significantly the metabolic activity in
the chondrocyte culture of the osteoarthrotic cartilage. Indometacin
in the concentration 10[^-5] and 10[^-6] mol/l increased the level
of incorporation. Tiaprofenic acid and tropesin at the lowest
concentration 10[^-7] mol/l (approaching the therapeutic level)
significantly increased incorporation of the labelled sulphate
and prolin into chondrocytes from the osteoarthrotic cartilage.
Key words: non-steroidal antirheumatic drugs biosynthesis,
extracellular matrix, chondrocytes, osteoarthrotic cartilage.
pp. 193-196
SILICONE ARTHROPATHY
J. ROVENSKY, M. STANCIKOVA, M. BROZMAN¹, K. BOSMANSKY, F. MALIS
Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
¹Klinika plastickej chirurgie, Bratislava
Riaditel: prof. MUDr. M. Brozman, DrSc.
Suhrn
Silikonove implantaty sa pomerne casto pouzivaju v plastickej
chirurgii. Silikon vsak nie je biologicky inertna latka. Moze
vyvolat zapalovu reakciu a proliferaciu fibroblastov s naslednou
zvysenou depoziciou kolagenu. Nemozno hovorit vzdy o zapalovej
reakcii, ale skor o reakcii organizmu na cudzorodu latku.
Relativne riziko vyvoja systemovych chorob spojiva, najma systemovej
sklerozy, sa zvysuje po aplikacii silikonovych implantatov u osob
s predispoziciou imunitneho systemu. Problemom vyjasnenia pricinneho
vztahu medzi vyvojom autoimunitneho ochorenia a implantaciou silikonovej
protezy je pomerne dlhe obdobie (1-20 rokov) medzi zavedenim implantatu
a vyvojom choroby.
Odstranenie silikonovej protezy ma niekedy priaznivy vplyv na
vyvoj choroby, opisali sa vsak aj pripady systemovej sklerozy,
ked ani odstranenie implantatu nezastavilo vyvoj choroby.
V praci sa diskutuje o moznostiach vyvoja autoimunitnych chorob
po implantacii silikonovych protez, pricom sa osobitny doraz kladie
na podrobne anamnesticke, fyzikalne a laboratorne vysetrenie buducej
nositelky z hladiska vyskytu autoimunitnych chorob, syndromov
a symptomov v snahe predist moznemu, dodatocnemu vyvoju tychto
chorob.
Klucove slova: silikonove implantaty, autoimunitne choroby,
tvorba protilatok.
Summary
Silicone implants are relatively frequently used in the plastic
surgery. Silicon, however, is not a biologically inert substance.
It can induce an inflammatory reaction and proliferation of fibroblasts
with a subsequent deposition of collagen. It is not possible to
speak always about an inflammatory reaction, but rather about
a response of the organism to a foreign substance.
The relative risk of the development of systemic connective tissue
diseases, especially systemic sclerosis increases after application
of silicon implants in the persons with predisposition of the
immune system. The problem of clarification of the causal relationship
between the development of the autoimmune disease and the implantation
of silicon prosthesis lies in a relatively long period (1-20 years)
between the implantation and the onset of the disease.
The removal of the silicon prosthesis has sometimes a favourable
influence on development of the disease, but there were described
also cases of systemic sclerosis, where even the implant removal
did not stop progression of the disease.
In the study the possibilities of the development of autoimmune
diseases after silicon prostheses implantations are discussed,
and a special emphasis is laid on detailed anamnestic, physical
and laboratory examinations of the future carrier from the point
of view of the autoimmune diseases, syndromes and symptoms, with
the aim to prevent a possible subsequent development of these
diseases.
Key words: silicone implants, autoimmune diseases, antibodies
production.
pp. 197-201
PATHOLOGIC CHANGES OF THE AORTA AND HEART VALVES IN ANKYLOSING SPONDYLITIS PATIENTS
J. BENACKA¹, F. MALIS², D. ZLNAY²
¹Interne oddelenie NsP, Piestany
Primar: MUDr. J. Letkovsky
²Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
Suhrn
Ciel prace: Podat uceleny prehlad o strukturalnych a funkcnych
zmenach steny aorty, aortalnej chlopne a mitralnej chlopne u pacientov
s ankylozujucou spondylitidou (AS).
Obsah prace: V uvode su zakladne udaje o historickom vyvoji
poznatkov a podrobne zhrnutie udajov z literatury o histologickych
zmenach struktur srdca u pacientov s AS. Dalsi text sa venuje
udajom z literatury o strukturalnych zmenach steny aorty, aortalnej
a mitralnej chlopne a o vplyve strukturalnych zmien na funkciu
chlopni a na vyskyt moznych komplikacii.
Udaje z literatury autori porovnavaju s vlastnymi zisteniami,
zaroven hladaju vysvetlenie pre rozdiely v literature suvisiace
s prevalenciou a zavaznostou zmien, ako aj ich vztahom k zakladnemu
ochoreniu.
Zavery: Polovica pacientov s AS ma klinicky malo vyznamne,
vcelku charakteristicke, nie vsak specificke zmeny na srdci a
na aorte. Najvhodnejsou metodou na ich detekciu je echokardiografia.
Treba na ne mysliet pri diferencialnej diagnostike kardialnych
tazkosti pacientov s AS.
Klucove slova: echokardiografia, ankylozujuca spondylitida,
choroby aorty, mimoklbove zmeny, m. Bechterew, bump.
Summary
Aim of work: To provide a comprehensive review of the structural
and functional changes of the aorta wall, aortal valve and mitral
valve in ankylosing spondylitis (AS) patients.
Content of the work: In the introduction, there are basic
data about the historical development of the knowledge and a detailed
summary of the data from the literature on histologic changes
of the heart structures in patients with AS. In the further part
the literature data on structural changes of the aorta wall, aortal
and mitral valve and on their influence on the function of the
valves and the occurrence of possible complications are summarized.
The authors compare the data from the literature with their own
findings, looking for the reasons for the differences in the literature
related to the prevalence and significance of the changes, as
well as their relation to the basic disease.
Conclusion: In half of the patients with AS the changes
on the heart and the aorta are clinically little important, as
a rule chraracteristic, but not specific. The most suitable method
for their detection is echocardiography. It is necessary to think
about them in the differential diagnostics of cardiac difficulties
of patients with AS.
Key words: echocardiography, ankylosing spondylitis, diseases
of the aorta, m. Bechterew, bump.
pp. 203-208
INHIBITORS OF ANGIOTENSIN-CONVERTING ENZYME IN THE THERAPY OF ANKYLOSING SPONDYLITIS
R. STANCIK, J. LUKAC
Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
Suhrn
Inhibitory angiotenzin-konvertujuceho enzymu (ACE), ktorych najvyznamnejsimi
predstavitelmi su captopril a enalapril, boli zavedene povodne
ako antihypertenziva v 70. rokoch. Neskorsie vyskumy priniesli
zaujimave poznatky o moznostiach vyuzitia tychto lieciv aj v reumatologii.
Ukazuje sa, ze inhibitory ACE maju svoj vyznam pri dlhodobej liecbe
tak klbovych chorob, ako aj oblickoveho postihnutia pri reumatickych
ochoreniach, pricom k nespornym vyhodam tejto skupiny liekov patri
aj dobra tolerancia. Pre ich dokladnejsie zhodnotenie vsak bude
treba este doplnit kvalitne porovnavacie studie.
Klucove slova: angiotenzin II, angiotenzin-konvertujuci
enzym, liecba reumatickych chorob, captopril, enalapril.
Summary
The inhibitors of angiotensin-converting enzyme (ACE), of which
the most important representatives are captopril and enalapril,
were introduced as antihypertensives in the 1970s. The later research
brought interesting findings about the possibilities of their
use also in rheumatology. It has been shown that the ACE inhibitors
have their place in the long-term therapy of the diseases of joints
as well as of the kidney involvement in the rheumatic diseases.
A great advantage of this group of drugs is also their good tolerance.
For a more through evaluation, however, it will be necessary to
perform further comparative studies of high quality.
Key words: angiotensin II, angiotensin-converting enzyme,
therapy of the rheumatic diseases, captopril, enalapril.
pp. 209-211
A CASE OF A SIMULTANEOUS OCCURRENCE OF RELAPSING POLYCHONDRITIS AND PSORIATIC ARTHRITIS
H. RAFFAYOVA, J. ROVENSKY
Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
Suhrn
U 38-rocneho pacienta sme opisali sucasny vyskyt benignej formy
relabujucej polychondritidy (r.p.) a nepriaznivo prebiehajucej
psoriatickej artritidy (p.a.). Ochorenie sa zacalo roku 1986 erytrodermickou
formou psoriazy, ku ktorej sa po dvoch rokoch pridruzili prejavy
relabujucej polychondritidy (sedlovity nos, zhrubnute a deformovane
usnice). Napokon v obdobi poslednych dvoch rokov nachadzame v
klinickom obraze artriticky syndrom.
Ochorenie (r.p. a p.a.) sa podarilo priaznivo ovplyvnit kombinovanou
imunosupresivnou liecbou (spociatku sa podaval sucasne metotrexat
s prednizonom, neskor cyklosporin s prednizonom).
Dosial sa opisali iba 3 podobne pripady. Nasou kazuistikou sme
chceli prispiet nielen k opisu sucasneho vyskytu dvoch nozologickych
reumatologickych jednotiek (r.p. a p.a.), ale poukazat aj na sucasne
terapeuticke moznosti ich ovplyvnenia.
Klucove slova: relabujuca polychondritida, psoriaticka
artritida.
Summary
In a 38-year old patient a simultaneous occurrence of benign form
of relapsing polychondritis (r.p.) and psoriatic arthritis (p.a.)
with an infavourable course was described. The disease started
in 1986 with an erythrodermic form of psoriasis to which after
two years manifestations of relapsing polychondritis (saddle nose,
thickened and deformed ear lobes) were associated. In the period
of the last two years we have noted in the clinical picture the
arthritic syndrome.
The disease (r.p. and p.a.) was successfully influenced by combined
immunosuppressive treatment (at the beginning simultaneous administration
of methotrexate and prednisone, later cyclosporine and prednisone).
Up to now, only 3 similar cases have been described. Our case-report
is a small contribution not only to the description of a simultaneous
accurrence of the two nosologic rheumatologic entities (r.p. and
p.a.), but it also describes current therapeutic possibilities
of their influencing.
Key words: relapsing polychondritis, psoriatic arthritis.
pp. 213-215
KIDNEY TRANSPLANTATION IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS
P. POPRAC, J. BREZA¹, J. LUKAC, J. ROVENSKY, A. KOVAC², K. KOHLER³
Vyskumny ustav reumatickych chorob, Piestany
Riaditel: prof. MUDr. J. Rovensky, DrSc.
¹Urologicka klinika Dererovej nemocnice, Bratislava
Prednosta: prof. MUDr. M. Hornak, DrSc.
²I. interna klinika IVZ, Bratislava
Prednosta: doc. MUDr. M. Pavlovic, CSc.
³Nemocnica s poliklinikou, Presov
Riaditel: MUDr. P. Chudik
Suhrm
Autori referuju o priebehu ochorenia a o jeho liecbe transplantaciou
oblicky u dvoch mladych zien so systemovym lupus erythematosus,
s "end stage renal disease", ktory sa rozvinul po porode.
Obe pacientky boli nasledne zaradene do liecby chronickou intermitentnou
hemodialyzou. Transplantacia sa uskutocnila po 31 a 16 mesiacoch
dialyzy. V pooperacnej periode sa liecili prednizonom a cyklosporinom
A. Rejekcne epizody sa liecili pulznou metylprednizolonovou liecbou
a antitymocytarnym globulinom. Oba transplantaty v potransplantacnom
odbobi nevyzadovali podpornu hemodialyzu. Jedna pacientka exitovala
po 3 mesiacoch na sepsu, u druhej pacientky s pozitivnymi antikardiolipinovymi
protilatkami doslo v 4. mesiaci po operacii k tromboze renalnej
arterie a musela sa urobit graftektomia. Pacientka pokracuje v
dialyzacnej liecbe.
Klucove slova: oblicka, transplantacia, systemovy lupus
erythematosus, hemodialyza, prednizon, cyklosporin.
Summary
The authors present the course of disease and its therapy by kidney
transplantation in two young women with systemic lupus erythematosus
and "end stage renal disease" developed after the childbirth.
Subsequently, in both women chronic intermittent dialysis was
introduced. The transplantation was performed after 31 and 16
months of dialysis. In the postoperative periode they were treated
with prednisone and cyclosporine A. The rejection episodes were
treated by pulse methylprednisolone therapy and antithymocytar
globulin. Neither graft in the postoperative period required supportive
hemodialysis. One patient existed after 3 months due to sepsis,
in the other patient with positive anticardiolipin antibodies
at month IV after the surgery thrombosis of renal arthery occurred
and graftectomy had to be performed. The patient continues to
be on dialysis therapy.
Key words: kidney, transplantation, systemic lupus erythematosus,
hemodialysis, prednisone, cyclosporine.
pp. 217-220